Anti-Human CEACAM5 Recombinant Antibody (A5B7) (CAT#: TAB-408LC)
Recombinant monoclonal antibody to CEACAM5. A5B7 is a mouse monoclonal antibody that can be potentially used in the treatment of cancer.
Specific Inquiry
SDS-PAGE
Figure 1 Anti-Human CEACAM5 Recombinant Antibody (TAB-408LC) in SDS-PAGE
SDS-PAGE analysis of TAB-408LC in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions.
SEC-HPLC
Figure 2 Anti-Human CEACAM5 Recombinant Antibody (TAB-408LC) in SEC-HPLC
The purity of TAB-408LC was 96.91% as determined by SEC-HPLC.
WB
Figure 3 Anti-Human CEACAM5 Recombinant Antibody (TAB-408LC) in WB
Western blot analysis of TAB-408LC was performed by loading human CEACAM5 protein, His tag.
ELISA
Figure 4 Anti-Human CEACAM5 Recombinant Antibody (TAB-408LC) in ELISA
ELISA analysis of TAB-408LC was performed by coating with human CEACAM5 protein (His tag). Then blocked with BSA and incubated with TAB-408LC. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Mouse
- Specificity
- Human
- Clone
- A5B7
- Related Disease
- Cancer
Product Property
- Preservative
- Human
- Storage
- CEA (carcinoembryonic antigen)
Target
- Alternative Names
- CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100;
- Gene ID
- 1048
- UniProt ID
- A0A024R0K5
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-080 | Recombinant Anti-human CEACAM5 VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-002LC-VHH | Anti-Human CEACAM5 Recombinant Antibody(VHH library) | SPR, FC, ELISA | Single domain antibody |
| HPAB-0204-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0204-FY) | ADCC, ELISA | llama VHH |
| HPAB-0205-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0205-FY) | ADCC, E | llama VHH |
| HPAB-1024WJ | Recombinant Camelid Anti-CEACAM5 Single Domain Antibody | SPR, Inhib | Camelid VHH |
Customer Reviews and Q&As
Customer Review
Q&As
Highly reliable reagent
We used A5B7 in several immunohistochemistry experiments, and it provided consistent staining patterns. The detailed staining allowed us to draw meaningful conclusions regarding CEACAM5 expression.
User-friendly datasheet
The accompanying datasheet was very comprehensive and provided all necessary instructions for use, storage, and dilution. It eased the setup of our experiments significantly.
Efficient CEACAM5 detection
In our immunofluorescence studies, A5B7 provided bright and distinct localization of CEACAM5, helping us map its distribution accurately within the samples.
M**k
Excellent for WB
Excellent for WB
We have utilized the Anti - Human CEACAM5 Recombinant Antibody (A5B7) in Western blotting (WB) experiments on protein lysates from human colon polyps, metastatic colorectal cancer tissues, and normal colon epithelial cells. The antibody showed outstanding sensitivity. It clearly detected CEACAM5, even at low expression levels, and revealed distinct band patterns. This was extremely useful for studying CEACAM5 - related signaling pathways in colorectal cancer.
01-Mar-25
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-408LC, RRID: AB_3111927)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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